Test name: Folate/Folic Acid
Order Code: FOL
Preferred Specimen: 2mL serum in a Serum Separator Tube (SST or Tiger-top)
CPT Code: 82746
Collection: Allow SST to clot in an upright position for 30 min, then centrifuge sample within 2 hours of collection. Refrigerate.
Special Instructions and Considerations: Hemolysis may significantly increase folate values due to high concentrations of folate in red blood cells. Therefore, hemolyzed samples are not suitable for use in this assay. Samples should not be taken from patients receiving therapy with high biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin administration. It is contraindicated to measure samples of patients receiving therapy with certain pharmaceuticals, e.g. methotrexate or leucovorin, because of the cross-reactivity of folate binding protein with these compounds. Samples with extremely high total protein concentrations (e.g. patients suffering from Waldenstrӧm’s macroglobulinemia) are not suitable for use in this assay, since they may lead to the formation of protein gel in the assay cup.
Transport Temperature: Refrigerated
Specimen Stability: 2 hours under room temperature; 2 days refrigerated
Causes for Rejection: Hemolysis