Unsaturated Iron Binding Capacity
Test name: Unsaturated Iron Binding Capacity
Order Code: UIBC
Preferred Specimen: 2mL serum in a Serum Separator Tube (SST or Tiger-top)
CPT Code: 83550
Collection: Allow SST to clot in an upright position for 60 min, then centrifuge sample within 2 hours of collection. Refrigerate.
Special Instructions and Considerations: Contamination with erythrocytes will elevate results, because the analyte level in erythrocytes is higher than in normal sera. The level of interference may be variable depending on the content of analyte in the lysed erythrocytes. Complexing anticoagulants such as ETDA, oxalate, and citrate must not be used. Oxytetracycline causes artificially high UIBC values at the tested drug level. Pathologically high levels of albumin (7 g/dL) decrease the apparant UIBC value significantly. If the patient’s serum iron exceeds the binding capacity of the transferrin, a negative UIBC value will result. In patients treated with iron supplements or metal-binding drugs, the drug-bound iron may not properly react in the test, resulting in falsely low values. Any drug containing deferoxamine will interefere with the UIBC assay.
Transport Temperature: Refrigerated
Specimen Stability: 4 days under room temperature; 7 days refrigerated
Causes for Rejection: moderate or greater hemolysis; moderate or greater lipemia